Part:BBa_K3190200:Design

GPA1:G Protein Alpha subunit

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The coding sequence for the yeast endogenous Gα i.e. GPA1 was used to make the chimera of GPA1-Gαs chimera and GPA1-Gαi chimera. These chimera acts as a adaptor molecule to bridge the human receptors to the endogenous beta subunit Ste4p, and gamma subunit Ste18p.

Source

S. cerevisiae